异丙肾上腺素对大鼠C6胶质瘤细胞β-肾上腺素能受体的剂量依赖性下调作用。

Dose-dependent down-regulation of beta-adrenoceptors by isoproterenol in rat C6 glioma cells.

作者信息

Bieck P R, Duhl D M, Eiring A, Gillespie D D, Manier D H, Sulser F

机构信息

Department of Psychiatry, Vanderbilt University School of Medicine, Nashville, TN 37232.

出版信息

Eur J Pharmacol. 1992 Feb 13;225(2):171-4. doi: 10.1016/0922-4106(92)90098-g.

DOI:10.1016/0922-4106(92)90098-g

PMID:1312942

Abstract

Nano- and micromolar isoproterenol concentrations were compared by studying cyclic AMP, beta-adrenoceptor density and beta 1-adrenoceptor mRNA in rat C6 glioma cells. 1 microM isoproterenol significantly changed all parameters at 15-30 min. The beta 1-antagonist metoprolol attenuated the response. No effects of nanomolar isoproterenol on these early changes were observed, although the density of beta-adrenoceptors was significantly reduced beginning at 12 h. The results indicate a different process for beta-adrenoceptor desensitization in C6 cells following physiologically low agonist concentrations.

摘要

通过研究大鼠C6胶质瘤细胞中的环磷酸腺苷（cAMP）、β - 肾上腺素能受体密度和β1 - 肾上腺素能受体mRNA，比较了纳摩尔和微摩尔浓度的异丙肾上腺素。1微摩尔异丙肾上腺素在15 - 30分钟时显著改变了所有参数。β1拮抗剂美托洛尔减弱了该反应。尽管从12小时开始β - 肾上腺素能受体的密度显著降低，但未观察到纳摩尔浓度的异丙肾上腺素对这些早期变化有影响。结果表明，在生理低激动剂浓度作用下，C6细胞中β - 肾上腺素能受体脱敏的过程有所不同。