Gabaldón J A, Cascales J M, Morias S, Maquieira A, Puchades R
Centro Tecnológico, Nacional da la Conserva, Murcia, Spain.
Food Addit Contam. 2003 Aug;20(8):707-15. doi: 10.1080/0265203031000152442.
A dipstick assay for the simultaneous determination of atrazine and carbaryl in vegetable samples was developed. The analytical method involved a fast extraction procedure followed by a multi-strip membrane test. The assay took 10 min, with a detection limit (naked eye) of 10 and 200 micro g l(-1) for atrazine and carbaryl, respectively. The cross-reactivities to related compounds tested were negligible except for propazine. Quantification of the pesticides was carried out by measuring the dot colour with a spectrophotometer. IC(50) values (inhibition constant at 50% of the maximum binding) of 2.04 microg l(-1) for atrazine and 92.8 microg l(-1) for carbaryl were achieved by this approach. Vegetable samples were extracted with MeOH. The proposed methodology was used to analyse atrazine and carbaryl and check for compliance with European Union maximum residue levels for vegetables. Recoveries (75-105%) were in agreement with those obtained by GC/MS or HPLC. Standard curves using 25% methanol/75% TBS were used for food sample assays using a multi-analyte dipstick. IC(50) values were 9.2 microg kg(-1) for atrazine and 179.2 microg kg(-1) for cabaryl.
开发了一种用于同时测定蔬菜样品中莠去津和西维因的试纸条检测方法。该分析方法包括快速萃取步骤，随后进行多试纸条膜测试。该检测耗时10分钟，莠去津和西维因的检测限（肉眼）分别为10和200微克/升。除了扑灭津外，对所测试的相关化合物的交叉反应可忽略不计。通过用分光光度计测量斑点颜色对农药进行定量。通过这种方法获得的莠去津的IC50值（最大结合量的50%时的抑制常数）为2.04微克/升，西维因的IC50值为92.8微克/升。蔬菜样品用甲醇萃取。所提出的方法用于分析莠去津和西维因，并检查是否符合欧盟蔬菜最大残留限量。回收率（75 - 105%）与通过气相色谱/质谱联用仪或高效液相色谱法获得的回收率一致。使用25%甲醇/75%三羟甲基氨基甲烷缓冲盐的标准曲线用于使用多分析物试纸条的食品样品检测。莠去津的IC50值为9.2微克/千克，西维因的IC50值为179.2微克/千克。
