Wauters Georges, Avesani Véronique, Laffineur Kim, Charlier Jacqueline, Janssens Michèle, Van Bosterhaut Bernard, Delmée Michel
University of Louvain, Faculty of Medicine, Microbiology Unit, B-1200 Brussels, Belgium.
Centre Hospitalier de Mouscron, 7700 Mouscron, Belgium.
Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1321-1325. doi: 10.1099/ijs.0.02513-0.
Three strains of coryneform rods isolated from clinical samples and one of environmental origin exhibited phenotypic and chemotaxonomic properties characteristic of the genus Brevibacterium and their 16S rRNA gene sequences were closely related (98.5-99.0 %) to that of Brevibacterium otitidis. However, DNA-DNA hybridization of one strain (CF87(T)) showed only 59.6 % relatedness to the type strain of B. otitidis, DSM 10718(T), and 75-82 % relatedness to the three other strains. The four strains could be differentiated from B. otitidis by cellular fatty acid composition and some phenotypic characteristics. These findings suggest that the four strains belong to a novel species, for which the name Brevibacterium lutescens sp. nov. is proposed. The type strain of B. lutescens is CF87(T) (=DSM 15022(T)=CCUG 46604(T)).
从临床样本中分离出的三株棒状杆菌和一株环境来源的棒状杆菌表现出短杆菌属的表型和化学分类学特性,它们的16S rRNA基因序列与耳炎短杆菌的序列密切相关(98.5 - 99.0%)。然而,其中一株菌株(CF87(T))与耳炎短杆菌模式菌株DSM 10718(T)的DNA - DNA杂交显示仅有59.6%的相关性,与其他三株菌株的相关性为75 - 82%。这四株菌株可通过细胞脂肪酸组成和一些表型特征与耳炎短杆菌区分开来。这些发现表明这四株菌株属于一个新物种,为此提出了新名称黄色短杆菌(Brevibacterium lutescens sp. nov.)。黄色短杆菌的模式菌株是CF87(T)(=DSM 15022(T)=CCUG 46604(T))。