Wauters Georges, Avesani Véronique, Charlier Jacqueline, Janssens Michèle, Vaneechoutte Mario, Delmée Michel
Microbiology Unit, University of Louvain, UCL/5490, Av. Hippocrate 54, B-1200 Brussels, Belgium.
J Clin Microbiol. 2005 Jun;43(6):2624-8. doi: 10.1128/JCM.43.6.2624-2628.2005.
Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.
通过16S rRNA基因测序对从比利时临床样本中分离出的86株诺卡菌菌株进行了鉴定。83株(96%)菌株仅属于6种诺卡菌:豚鼠耳炎诺卡菌(38株[44%])、新星诺卡菌(19株[22%])、赛氏诺卡菌(13株[15%])、巴西诺卡菌(6株[6.9%])、脓肿诺卡菌(5株[5.8%])和少食诺卡菌(2株[2.3%])。开发了一组包含9项传统和酶学检测的方法,用于快速鉴定本次调查中分离出的最常见菌种。发现吡咯烷酮氨基肽酶、γ-谷氨酰氨基肽酶、α-甘露糖苷酶和α-葡萄糖苷酶具有高度鉴别性,可用于制定鉴定方案。