Makoc Z, Trojan S, Vorel F
Physiol Bohemoslov. 1976;25(1):23-9.
Enzymatic activities were determined in the prosencephalon of rats which had been exposed repeatedly for 8 hrs, either from the age of 1 to 17 days or in adulthood, to an altitude of 7,000 m in a barometric chamber (up to a total of 104 hrs). The activity of enzymes was assayed 20 hrs after the last exposure. The results were compared with values obtained 20 hrs after a single 8-hour exposure to a stimulated altitude of 7,000 m in 17-day-old and adult rats. In young rats a single exposure to hypoxia, the most elevated was the activity of LDH and isoCDH, while GPT and CPK were decreased. After repeated hypoxia, the most significant increasw was noted in the activity of PK and again a decrease in GPT and CPK. In adulthood, a single exposure to hypoxia causes the greatest increase in CPK, while LDH and GOT are reduced. Following repeated hypoxia, none of the enzyme activities were increased by more than 20%, while LDH, GOT, GIDH and CPK were again lower. It is concluded from these results that a single exposure to hypoxia increases anaerobic glycolysis in the immature nervous tissue and improves its oxygen utilization. The relationship between glycid and amino acid metabolism are not appreciably altered. On the other hand, a general reduction of bioenergetics and biosynthesis occurs in adulthood. Repeated hypoxia, on the contrary, has similar after-effects in both age groups, namely inhibition of aerobic metabolism and of the relations between glycid and amino acid metabolism.
对出生1至17天或成年后反复暴露于气压舱中7000米高度8小时（累计达104小时）的大鼠前脑进行酶活性测定。在最后一次暴露后20小时测定酶活性。将结果与17日龄和成年大鼠单次暴露于模拟7000米高度8小时后20小时获得的值进行比较。在幼鼠中，单次暴露于低氧环境下，乳酸脱氢酶（LDH）和异柠檬酸脱氢酶（isoCDH）活性升高最为明显，而谷丙转氨酶（GPT）和肌酸磷酸激酶（CPK）活性降低。反复低氧后，丙酮酸激酶（PK）活性显著增加，GPT和CPK活性再次降低。在成年大鼠中，单次暴露于低氧环境会使CPK增加最多，而LDH和谷草转氨酶（GOT）降低。反复低氧后，没有一种酶活性增加超过20%，而LDH、GOT、谷氨酸脱氢酶（GIDH）和CPK再次降低。从这些结果可以得出结论，单次暴露于低氧环境会增加未成熟神经组织中的无氧糖酵解并改善其氧利用。糖代谢和氨基酸代谢之间的关系没有明显改变。另一方面，成年期生物能量学和生物合成普遍降低。相反，反复低氧在两个年龄组中具有相似的后效应，即抑制有氧代谢以及糖代谢和氨基酸代谢之间的关系。
