Pater A, Belaguli N S, Gardner H A, Mithal A, Pater M M
Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Virology. 1992 May;188(1):369-72. doi: 10.1016/0042-6822(92)90767-j.
The establishment of transformation of primary rodent cells by human papillomavirus (HPV) type 16 DNA requires glucocorticoid hormones (Pater et al., Nature 335, 832-835, 1988). Here we provide evidence by mutational analysis that, in the context of the hormone-regulated HPV 16 promoter/enhancer, the only protein coding sequences of HPV 16 required are those of the E7 gene. Moreover, additional sequences adjacent to the 3' end of E7 coding sequences are also essential for the establishment of the transformed phenotype. Splice donor sites, especially an E7 ORF 3' proximal one, are implicated for this cis-acting function, since specific deletion mutations of these splice sites greatly or completely reduced the frequency of transformation and the level of E7 RNA.
人乳头瘤病毒16型（HPV）DNA对原代啮齿动物细胞的转化需要糖皮质激素（Pater等人，《自然》335卷，832 - 835页，1988年）。在此，我们通过突变分析提供证据表明，在激素调节的HPV 16启动子/增强子背景下，HPV 16所需的唯一蛋白质编码序列是E7基因的序列。此外，E7编码序列3'端相邻的其他序列对于转化表型的建立也是必不可少的。剪接供体位点，尤其是E7开放阅读框3'近端的一个，与这种顺式作用功能有关，因为这些剪接位点的特异性缺失突变极大地或完全降低了转化频率和E7 RNA水平。
