肌醇（1,3,4,5）四磷酸促进分离的小鼠泪腺细胞中钙（2 +）依赖性氯电流的持续激活。
Ins(1,3,4,5)P4 promotes sustained activation of the Ca(2+(-dependent Cl- current in isolated mouse lacrimal cells.
作者信息
Smith P M
机构信息
Department of Physiology, University of Liverpool, U.K.
出版信息
Biochem J. 1992 Apr 1;283 ( Pt 1)(Pt 1):27-30. doi: 10.1042/bj2830027.
Abstract
Infusion of 50 microM-Ins(1,3,4,5)P4 in addition to 500 microM-Ins(1,4,5)P3 into mouse lacrimal cells via a patch-clamp pipette promoted sustained activation of the Ca(2+)-dependent Cl- current, which could not be achieved with 500 microM-Ins(1,4,5)P3 alone. It has been proposed that Ins(1,3,4,5)P4 facilitates Ca2+ influx in the presence of Ins(1,4,5)P3 [Morris, Gallacher, Irvine & Petersen (1987) Nature (London) 330, 653-655], but a subsequent study in mouse lacrimal cells [Bird, Rossier, Hughes, Shears, Armstrong & Putney (1991) Nature (London) 352, 162-165] showed that a high concentration of Ins(1,4,5)P3 could mobilize both intra- and extra-cellular Ca2+ in the absence of Ins(1,3,4,5)P4. My data confirm these findings, but also show that Ins(1,3,4,5)P4 can stimulate additional Ca2+ influx even when the Ins(1,4,5)P3-dependent intracellular Ca2+ pools have been depleted.
摘要
通过膜片钳移液管向小鼠泪腺细胞中注入500微摩尔肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）的同时，再注入50微摩尔肌醇-1,3,4,5-四磷酸（Ins(1,3,4,5)P4），可促进钙依赖性氯离子电流的持续激活，而单独注入500微摩尔Ins(1,4,5)P3则无法实现这一点。有人提出，在存在Ins(1,4,5)P3的情况下，Ins(1,3,4,5)P4有助于钙离子内流[莫里斯、加拉赫、欧文和彼得森（1987年），《自然》（伦敦）330, 653 - 655]，但随后在小鼠泪腺细胞中的一项研究[伯德、罗西耶、休斯、希尔斯、阿姆斯特朗和普特尼（1991年），《自然》（伦敦）352, 162 - 165]表明，在不存在Ins(1,3,4,5)P4的情况下，高浓度的Ins(1,4,5)P3可动员细胞内和细胞外的钙离子。我的数据证实了这些发现，但也表明即使依赖Ins(1,4,5)P3的细胞内钙库已经耗尽，Ins(1,3,4,5)P4仍能刺激额外的钙离子内流。
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