Vasta V, Smith C J, Calvo J, Belfrage P, Manganiello V C
Laboratory of Cellular Metabolism, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1992 Mar 31;183(3):1070-5. doi: 10.1016/s0006-291x(05)80299-2.
The cGI PDE in particulate fractions of differentiated adipocytes (but not control 3T3-L1 fibroblasts) was cross-reactive with a polyclonal antibody raised against the bovine adipose cGI PDE. The 3T3-L1 adipocyte cGI PDE is a 135 kDa protein which is phosphorylated in 32P-labeled cells in response to beta-agonist or insulin. These results indicate that the 3T3-L1 cGI PDE is similar in structure and hormonal regulation to the analogous enzyme in the rat adipocyte.
分化脂肪细胞（而非对照的3T3-L1成纤维细胞）微粒部分中的cGI磷酸二酯酶与针对牛脂肪cGI磷酸二酯酶产生的多克隆抗体发生交叉反应。3T3-L1脂肪细胞cGI磷酸二酯酶是一种135 kDa的蛋白质，在32P标记的细胞中，它会因β-激动剂或胰岛素而发生磷酸化。这些结果表明，3T3-L1 cGI磷酸二酯酶在结构和激素调节方面与大鼠脂肪细胞中的类似酶相似。
