兔卵巢中II型环磷酸腺苷依赖性蛋白激酶的调节亚基是RIIβ亚型。
The regulatory subunit of the type II cAMP-dependent protein kinase in rabbit ovaries is the RII beta isoform.
作者信息
Jackiw V, Hunzicker-Dunn M
机构信息
Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, IL 60611.
出版信息
Biochem Biophys Res Commun. 1992 Apr 15;184(1):43-9. doi: 10.1016/0006-291x(92)91155-j.
Based on RII autophosphorylation, photoaffinity labeling with 8-N3[32P]cAMP, and Western blot analysis we have identified the RII isoform found in rabbit corpora lutea as RII beta. The RII beta subunit found in rabbit corpora lutea differs from the RII beta found in rat follicles and corpora lutea in that it migrates at Mr 52,500 on SDS-PAGE and shifts to Mr 53,000 when phosphorylated.
基于RII自身磷酸化、用8-N3[32P]cAMP进行光亲和标记以及蛋白质印迹分析，我们已鉴定出在兔黄体中发现的RII同工型为RIIβ。在兔黄体中发现的RIIβ亚基与在大鼠卵泡和黄体中发现的RIIβ不同，在于它在SDS-PAGE上的迁移分子量为52,500，磷酸化后迁移分子量变为53,000。
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