Pérez-Castrillón J L, Pérez-Arellano J L, García-Palomo J D, Jiménez-López A, De Castro S
Department of Medicine, University of Salamanca, Spain.
Immunopharmacology. 1992 Jan-Feb;23(1):57-61. doi: 10.1016/0162-3109(92)90009-2.
Intravenous drug abusers (IDA) normally show functional defects in monocyte activity, in particular their chemotactic response. The aim of the present work was to study the action of several opioids on monocyte chemotaxis. To do so, monocytes from healthy individuals were incubated with heroin and morphine at three different concentrations (10(-5) M, 10(-6) M and 10(-7) M), with the finding of a significant depression in monocyte chemotaxis in all cases. This alteration could be due to a receptor effect or, conversely, to a non-specific effect. Accordingly, in the second phase of the study, monocytes from controls were incubated with a selective agonist of mu receptors (DAGO) and a selective agonist of delta receptors (DPDPE). In both cases a decrease in chemotactic function was observed similar to that following incubation with morphine or heroin. Preincubation of the monocytes with naloxone prevented the depression induced by both specific agonists. These findings suggest that opioids play an important role in the depression of monocyte chemotaxis observed in IDA. The results also suggest the presence of mu and delta opiate receptors in the cells of the phagocytic mononuclear system.
静脉注射吸毒者（IDA）通常在单核细胞活性方面表现出功能缺陷，尤其是其趋化反应。本研究的目的是研究几种阿片类药物对单核细胞趋化性的作用。为此，将健康个体的单核细胞与海洛因和吗啡在三种不同浓度（10⁻⁵M、10⁻⁶M和10⁻⁷M）下孵育，结果发现在所有情况下单核细胞趋化性均显著降低。这种改变可能是由于受体效应，或者相反，是由于非特异性效应。因此，在研究的第二阶段，将对照组的单核细胞与μ受体选择性激动剂（DAGO）和δ受体选择性激动剂（DPDPE）孵育。在这两种情况下，均观察到趋化功能下降，类似于与吗啡或海洛因孵育后的情况。用纳洛酮对单核细胞进行预孵育可防止两种特异性激动剂诱导的趋化性降低。这些发现表明，阿片类药物在IDA中观察到的单核细胞趋化性降低中起重要作用。结果还表明，吞噬单核系统的细胞中存在μ和δ阿片受体。
