Riehl T E, Turk J, Stenson W F
Department of Gastroenterology, Jewish Hospital of St. Louis, MO.
J Lipid Res. 1992 Mar;33(3):323-31.
Monolayers of Caco-2 cells, a human enterocyte cell line, were incubated separately with 3H8-labeled preparations of three different lipid mediators of inflammation: 5-hydroxyeicosatetraenoic acid, 12-hydroxyeicosatetraenoic acid, and leukotriene B4. Both [3H8]5-hydroxyeicosatetraenoic and [3H8]12-hydroxyeicosatetraenoic acids were taken up and metabolized by Caco-2 cells, but [3H]leukotriene B4 remained unmetabolized in the incubation medium. [3H]5-hydroxyeicosatetraenoic acid was esterified into cellular phospholipids (15%) and triglycerides (4%) but did not undergo beta-oxidation. When [3H]12-hydroxyeicosatetraenoic acid was incubated with Caco-2 cells, 14% underwent two cycles of beta-oxidation to form [3H]8-hydroxyhexadecatrienoic acid, and 3% underwent three cycles of beta-oxidation to form [3H]6-hydroxytetradecadienoic acid, both of which were released into the media. [3H]12-Hydroxyeicosatetraenoic acid was also esterified into cellular phospholipids (13%), but none was esterified into cellular triglycerides.
人肠上皮细胞系Caco-2细胞单层分别与三种不同炎症脂质介质的3H8标记制剂孵育：5-羟基二十碳四烯酸、12-羟基二十碳四烯酸和白三烯B4。[3H8]5-羟基二十碳四烯酸和[3H8]12-羟基二十碳四烯酸均被Caco-2细胞摄取并代谢，但[3H]白三烯B4在孵育培养基中未被代谢。[3H]5-羟基二十碳四烯酸被酯化为细胞磷脂（15%）和甘油三酯（4%），但未进行β-氧化。当[3H]12-羟基二十碳四烯酸与Caco-2细胞孵育时，14%经历两个β-氧化循环形成[3H]8-羟基十六碳三烯酸，3%经历三个β-氧化循环形成[3H]6-羟基十四碳二烯酸，两者均释放到培养基中。[3H]12-羟基二十碳四烯酸也被酯化为细胞磷脂（13%），但没有被酯化为细胞甘油三酯。
