Vonèche V, Portetelle D, Kettmann R, Willems L, Limbach K, Paoletti E, Ruysschaert J M, Burny A, Brasseur R
Faculty of Agronomy, Gembloux, Belgium.
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3810-4. doi: 10.1073/pnas.89.9.3810.
Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-terminal segment of gp30 (fusion peptide) into the lipid bilayer in an oblique orientation, as predicted by computer conformational analysis, results in fusogenic capacities higher than insertion in a perpendicular or parallel orientation; and (iii) replacement of the BLV fusion peptide with its simian immunodeficiency virus counterpart does not modify the fusogenic capacity of the BLV glycoprotein.
通过痘苗病毒重组体表达修饰的牛白血病病毒（BLV）糖蛋白，并通过合胞体形成试验检测其融合能力。该分析表明：（i）BLV包膜糖蛋白gp51和gp30两者对于细胞融合都是必需的；（ii）如计算机构象分析所预测的，gp30的N端片段（融合肽）以倾斜方向插入脂质双层，导致其融合能力高于以垂直或平行方向插入；以及（iii）用猿猴免疫缺陷病毒的对应物替换BLV融合肽不会改变BLV糖蛋白的融合能力。
