Erdel M, Spiess E, Trefz G, Boxberger H J, Ebert W
Department of Clinical Chemistry and Bacteriology, Thoraxklinik Heidelberg, F.R.G.
Anticancer Res. 1992 Mar-Apr;12(2):349-59.
The human NSCLC cell lines HS-24 (squamous cell carcinoma) and SB-3 (metastasis derived from an adenocarcinoma) were investigated in respect to cell interactions, motility and invasive properties. HS-24 revealed high self adhesion capacity. Testing the interactions with collagens type I/III or IV, laminin and fibronectin by adhesion, non directional motility and haptotaxis assays, tight interactions and stimulation, particularly with collagen type I/III, was detected. Proteinase inhibitors (E64, Stefin A or leupeptin) revealed a slightly negative influence. Invasion in vitro of lung explants was reduced by leupeptin in a dose dependent manner and slightly increased by plasmin. SB-3 cells revealed low self adhesion. As judged from interaction with fibronectin, these cells have low integrin receptor concentrations and thus reduced adhesiveness to extracellular matrix. Collagen type I/III was inhibitory for undirectional motility and not permissive for haptotaxis. Therefore, it may play a restrictive role during the spread in vivo of these cells. Colonization of lung explants was low and was not influenced by cathepsin B proteinase inhibitors. The results emphasize a particular role of collagens for primary site tumor and metastasis development.
对人非小细胞肺癌细胞系HS-24（鳞状细胞癌）和SB-3（源自腺癌的转移瘤）的细胞相互作用、运动性和侵袭特性进行了研究。HS-24表现出高自黏附能力。通过黏附、非定向运动和趋触性试验检测其与I/III型或IV型胶原蛋白、层粘连蛋白和纤连蛋白的相互作用，发现存在紧密相互作用和刺激，尤其是与I/III型胶原蛋白的相互作用。蛋白酶抑制剂（E64、丝氨酸蛋白酶抑制剂A或亮抑酶肽）显示出轻微的负面影响。亮抑酶肽以剂量依赖方式降低肺外植体的体外侵袭能力，而纤溶酶则使其略有增加。SB-3细胞表现出低自黏附性。从与纤连蛋白的相互作用判断，这些细胞的整合素受体浓度较低，因此对细胞外基质的黏附性降低。I/III型胶原蛋白对非定向运动具有抑制作用，对趋触性不具有允许作用。因此，它可能在这些细胞的体内扩散过程中起限制作用。肺外植体的定植率较低，且不受组织蛋白酶B蛋白酶抑制剂的影响。结果强调了胶原蛋白在原发性肿瘤和转移发展中的特殊作用。
