Fairley C K, Chen S, Tabrizi S N, Quinn M A, McNeil J J, Garland S M
Department of Microbiology, Royal Women's Hospital, Carlton, Prahran, Australia.
J Infect Dis. 1992 Jun;165(6):1103-6. doi: 10.1093/infdis/165.6.1103.
Assessment of human papillomavirus (HPV) infection usually requires a speculum examination to collect genital specimens. A technique using tampons as a patient-administered method for the collection of specimens was studied by dot blot hybridization (HPV types 6, 11, 16, 18, 31, and 33) and polymerase chain reaction (PCR). Tampons and cervical scrapes were collected from 48 consecutive women attending a dysplasia clinic. Tampons provided a significantly larger pellet volume (P less than .002) and more DNA (P less than .01) than scrapes. There was a close correlation when samples were analyzed for the presence of HPV DNA. Using dot blot hybridization, 8 cervical scrapes (17%) and 9 tampons (19%) were positive for HPV DNA (90% correlation). By PCR, 35 cervical scrapes (73%) and 33 tampons (69%) were positive for HPV DNA (88% correlation). Thus, tampon specimens are an easy method for assessment of genital HPV infection.
人乳头瘤病毒(HPV)感染的评估通常需要进行窥镜检查以采集生殖器标本。通过斑点杂交(HPV 6、11、16、18、31和33型)和聚合酶链反应(PCR)研究了一种使用棉塞作为患者自行采集标本的方法。从48名连续就诊于发育异常诊所的女性中采集棉塞和宫颈刮片。棉塞提供的沉淀体积明显更大(P小于0.002),且DNA含量更多(P小于0.01)。对样本进行HPV DNA检测时,两者存在密切相关性。使用斑点杂交法,8份宫颈刮片(17%)和9份棉塞(19%)的HPV DNA呈阳性(相关性为90%)。通过PCR检测,35份宫颈刮片(73%)和33份棉塞(69%)的HPV DNA呈阳性(相关性为88%)。因此,棉塞标本是评估生殖器HPV感染的一种简便方法。