Sherson D, Nielsen H, Frederiksen J, Milman N, Struve-Christensen E, Petersen B N
Department of Pulmonary Medicine, Bispebjerg Hospital, Copenhagen, Denmark.
APMIS. 1992 May;100(5):408-14. doi: 10.1111/j.1699-0463.1992.tb00891.x.
Superoxide anion release (O2-) after stimulation with phorbol myristate acetate was measured in alveolar macrophages (AM) obtained by bronchoalveolar lavage and in blood monocytes from 47 patients with diffuse interstitial lung disease: idiopathic pulmonary fibrosis (N = 15), hypersensitivity pneumonitis (N = 7), pneumoconiosis (N = 6) and sarcoidosis (N = 19). Differential cell counts demonstrated a lymphocyte predominance in patients with hypersensitivity pneumonitis (HP) and sarcoidosis while the other groups had neutrophil predominance. No correlation between O2- activity in alveolar macrophages (AM) or blood monocytes (BM) compared to lung function (VC and diffusing capacity) could be demonstrated. Smoking pneumoconiotics had significantly decreased BM O2- release (1.25 +/- 0.25 (SEM) nmol/min/10(6) cells) and significantly increased AM/BM O2- ratios (2.04 +/- 0.26) compared to smokers with idiopathic pulmonary fibrosis (IPF) who had the following mean values: BM O2- release = 2.58 +/- 0.25 and AM/BM O2- ratio = 0.86 +/- 0.23. When matched for sex and smoking, a significantly increased AM/BM O2- ratio was seen among patients with HP (2.19 +/- 0.98) in comparison with patients who had sarcoidosis (0.40 +/- 0.18). Patients with either HP or pneumoconiosis had generally elevated AM O2- release and reduced BM O2- release. These results suggest that environmentally related interstitial lung disorders (HP and pneumoconiosis) may be associated with elevated AM O2- release relative to BM O2- release in comparison to non-environmentally related disorders (IPF or sarcoidosis).
用佛波酯肉豆蔻酸酯刺激后，对47例弥漫性间质性肺疾病患者支气管肺泡灌洗获得的肺泡巨噬细胞（AM）和血液单核细胞中的超氧阴离子释放（O2-）进行了测量，这些患者包括特发性肺纤维化（N = 15）、过敏性肺炎（N = 7）、尘肺（N = 6）和结节病（N = 19）。细胞分类计数显示，过敏性肺炎（HP）和结节病患者以淋巴细胞为主，而其他组以中性粒细胞为主。肺泡巨噬细胞（AM）或血液单核细胞（BM）中的O2-活性与肺功能（肺活量和弥散能力）之间未显示出相关性。与特发性肺纤维化（IPF）吸烟者相比，吸烟尘肺患者的BM O2-释放显著降低（1.25±0.25（SEM）nmol/分钟/10(6)个细胞），AM/BM O2-比值显著升高（2.04±0.26），IPF吸烟者的平均值如下：BM O2-释放 = 2.58±0.25，AM/BM O2-比值 = 0.86±0.23。在按性别和吸烟情况匹配后，与结节病患者（0.40±0.18）相比，HP患者的AM/BM O2-比值显著升高（2.19±0.98）。HP或尘肺患者的AM O2-释放通常升高，BM O2-释放降低。这些结果表明，与非环境相关疾病（IPF或结节病）相比，环境相关的间质性肺疾病（HP和尘肺）可能与AM O2-释放相对于BM O2-释放升高有关。
