Duchêne A M, Patte J, Gutierrez C, Chandler M
Molecular Genetics and Microbiology Unit (CNRS), Toulouse, France.
Gene. 1992 May 1;114(1):103-7. doi: 10.1016/0378-1119(92)90714-z.
We have developed a two-plasmid system for generating gene fusions between phoA and cloned genes encoding envelope proteins. The vector plasmid carries a temperature-sensitive replication system and can be rescued at high temperature by insertion of an IS1-based transposon carrying the ori region of pBR322 and a phoA gene lacking transcription and translation initiation signals. The vector plasmid also carries the transfer origin of the conjugative plasmid, F, permitting transfer into a suitable recipient strain. We have used this system in the analysis of the bla gene cloned from pBR322.
我们开发了一种双质粒系统，用于在phoA与编码包膜蛋白的克隆基因之间产生基因融合。载体质粒携带一个温度敏感型复制系统，通过插入一个基于IS1的转座子可在高温下拯救该系统，该转座子携带pBR322的ori区域和一个缺乏转录和翻译起始信号的phoA基因。载体质粒还携带接合质粒F的转移起始位点，允许转移到合适的受体菌株中。我们已将该系统用于分析从pBR322克隆的bla基因。
