Ohnishi S T, Sakamoto A, Ohnishi T, Ogawa R
Philadelphia Biomedical Research Institute, King of Prussia, PA 19406.
Prostaglandins Leukot Essent Fatty Acids. 1992 Mar;45(3):217-21. doi: 10.1016/0952-3278(92)90116-z.
The inhibition of lipid peroxidation by oligomeric derivatives synthesized from prostaglandin E1 (PGE1) and PGB2 was studied using two rat models. In an in vitro model, the brain was exposed to decapitation-ischemia, the cortex was removed and homogenized, and the formation of thiobarbituric acid reactive substances (TBAR) was measured after exposing the homogenate to in vitro reoxygenation either in the presence or absence of oligomers. It was found that these oligomers could inhibit lipid peroxidation, and that their activities were higher than that of superoxide dismutase (SOD). In an in vivo administration model, either the oligomer or the vehicle was injected i.p. 30 min before decapitation. The brain was exposed to decapitation-ischemia, the cortex was homogenized and exposed to 'in vitro' reoxygenation, after which TBAR value was determined. Ester-type compounds had a greater activity than free-acid type compounds in inhibiting lipid peroxidation. A possible mechanism of the protective effect of these oligomers in ischemia/reperfusion injury may be to scavenge oxygen free radicals.
利用两种大鼠模型研究了由前列腺素E1（PGE1）和前列腺素B2（PGB2）合成的低聚物衍生物对脂质过氧化的抑制作用。在体外模型中，将大鼠脑断头致缺血，取出皮层并匀浆，在存在或不存在低聚物的情况下，将匀浆进行体外复氧后，测定硫代巴比妥酸反应性物质（TBAR）的形成。结果发现，这些低聚物能够抑制脂质过氧化，且其活性高于超氧化物歧化酶（SOD）。在体内给药模型中，在断头前30分钟腹腔注射低聚物或赋形剂。将大鼠脑断头致缺血，取出皮层匀浆并进行“体外”复氧，然后测定TBAR值。酯型化合物在抑制脂质过氧化方面比游离酸型化合物具有更强的活性。这些低聚物对缺血/再灌注损伤具有保护作用的可能机制可能是清除氧自由基。
