Dedkov V S, Degtiarev S Kh
Prikl Biokhim Mikrobiol. 1992 Mar-Apr;28(2):309-13.
A simple technique is proposed for the detection of restriction endonucleases in Streptomyces and Nocardia cells. The analysis was performed directly in the cells collected from colonies cultivated on Petri dishes with an inoculation loop. The cells were treated with lysozyme, EDTA and Triton X-100. The lysates were tested for restriction endonucleases. The technique enables the detection of enzymes Nco I, Not I, Nru I, Sfr 3031, and Sfi I in the lysates of the respective strains-producers.
本文提出了一种用于检测链霉菌和诺卡氏菌细胞中限制性内切酶的简单技术。该分析直接在培养于培养皿上的菌落中，用接种环收集的细胞中进行。细胞用溶菌酶、乙二胺四乙酸（EDTA）和吐温X - 100处理。对裂解物进行限制性内切酶检测。该技术能够在相应的菌株 - 产生菌的裂解物中检测到Nco I、Not I、Nru I、Sfr 3031和Sfi I酶。
