Hou L, Takeuchi T
Biological Institute, Faculty of Science, Tohoku University, Sendai, Japan.
In Vitro Cell Dev Biol. 1992 May;28A(5):348-54. doi: 10.1007/BF02877058.
An attempt was made to culture neural crest cells of the turtle embryo in vitro. Trunk neural tubes from the St. 9/10 embryos were explanted in culture dishes. The developmental potency of the turtle neural crest cells in vitro was shown to be essentially similar to that of avian neural crest cells, although they seem to be more sensitive to melanocyte-stimulating hormone (MSH) stimulation. We describe conditions under which explanted neural tube gives rise to neural crest cells that differentiate into neuronal cells and melanocytes. The potency of melanocyte differentiation was found to vary according to the concentration of fetal bovine serum (FBS, from 5 to 20%). Melanization of neural crest cells cultured in the medium containing FBS and alpha-MSH was more extensive than those cultured with FBS alone, combinations of FBS and chick embryo extract, or turtle embryo extract. These culture conditions seem to be useful for the study of the developmental potency of the neural crest cells as well as for investigating local environmental factors.
人们尝试在体外培养龟胚胎的神经嵴细胞。将来自9/10期胚胎的躯干神经管外植于培养皿中。结果表明，龟神经嵴细胞在体外的发育潜能与鸟类神经嵴细胞基本相似，尽管它们似乎对促黑素细胞激素（MSH）刺激更为敏感。我们描述了外植神经管产生分化为神经元细胞和黑素细胞的神经嵴细胞的条件。发现黑素细胞分化的潜能会因胎牛血清（FBS）浓度（5%至20%）的不同而有所变化。在含有FBS和α-MSH的培养基中培养的神经嵴细胞的黑素化程度比单独用FBS、FBS与鸡胚提取物或龟胚提取物组合培养的神经嵴细胞更广泛。这些培养条件似乎有助于研究神经嵴细胞的发育潜能以及调查局部环境因素。
