Derby M A, Newgreen D F
Cell Tissue Res. 1982;225(2):365-78. doi: 10.1007/BF00214689.
Neural crest cells from quail embryos grown in standard culture dishes differentiate almost entirely into melanocytes within 4 or 5 days when chick embryo extract (CEE) or occasional lots of fetal calf serum (FCS) are included in the medium. Gel fractionation showed that the pigment inducing factor(s) present in these media is of high molecular weight (greater than 400K daltons). In the absence of CEE, the neural tube can also stimulate melanocyte differentiation. Culture medium supplemented by selected lots of FCS permits crest cell proliferation but little overt differentiation after up to 2 weeks in culture if the neural tube is removed within 18 h of explantation in vitro. Subsequent addition of CEE to such cultures promotes complete melanocyte differentiation. Crest cells from White leghorn chick embryos also differentiate into melanocytes in the presence of CEE, but do not survive well in its absence. Melanocyte differentiation of crest cells from both quail and chick embryos can by suppressed by culturing under a dialysis membrane, even in the presence of the neural tube and CEE, but neuronal differentiation appears greatly enhanced.
在标准培养皿中培养的鹌鹑胚胎神经嵴细胞，若培养基中含有鸡胚提取物（CEE）或偶尔的几批胎牛血清（FCS），则在4或5天内几乎完全分化为黑素细胞。凝胶分级分离显示，这些培养基中存在的色素诱导因子分子量较高（大于400千道尔顿）。在没有CEE的情况下，神经管也能刺激黑素细胞分化。如果在体外接种后18小时内移除神经管，添加选定批次FCS的培养基可使嵴细胞增殖，但在培养长达2周后几乎没有明显分化。随后向此类培养物中添加CEE可促进黑素细胞完全分化。在CEE存在的情况下，白来航鸡胚胎的嵴细胞也会分化为黑素细胞，但在没有CEE的情况下存活不佳。即使在存在神经管和CEE的情况下，通过在透析膜下培养，鹌鹑和鸡胚胎嵴细胞的黑素细胞分化也会受到抑制，但神经元分化似乎大大增强。
