一种用于检测人乳头瘤病毒16型（HPV16）和人乳头瘤病毒18型（HPV18）E7转化蛋白的酶联免疫吸附捕获试验。

An ELISA capture assay for the E7 transforming proteins of HPV16 and HPV18.

作者信息

Selvey L A, Dunn L A, Murray B, Tindle R W, Frazer I H

机构信息

Papillomavirus Research Unit, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Australia.

出版信息

J Virol Methods. 1992 May;37(2):119-27. doi: 10.1016/0166-0934(92)90039-g.

DOI:10.1016/0166-0934(92)90039-g

PMID:1317876

Abstract

ELISA capture assays were established for the E7 transforming proteins of HPV16 and HPV18, based on a range of previously characterised polyclonal and monoclonal antibodies. No cross-reactivity was observed in the ELISAs between HPV18 E7 and HPV16 E7. Immunoreactive E7 protein (iE7) was measured in a series of HPV-transformed cell lines, and ranged from 0.6 to 17.7 ng iE7/mg cell protein. iE7 was labile at 22 degrees C (t1/2 = 37 min) but relatively more stable at 4 degrees C (t1/2 = 210 min). HPV16 E7 protein at concentrations from 0.10 to 0.69 ng iE7/mg cell protein was detected in 5 of 13 smears from women with abnormal cervical cytology. Assay of E7 protein may play a role in the detection of HPV-induced cervical lesions with malignant potential.

摘要

基于一系列先前已鉴定的多克隆抗体和单克隆抗体，建立了针对人乳头瘤病毒16型（HPV16）和人乳头瘤病毒18型（HPV18）E7转化蛋白的酶联免疫吸附捕获试验。在酶联免疫吸附试验中未观察到HPV18 E7和HPV16 E7之间存在交叉反应。在一系列HPV转化的细胞系中检测到免疫反应性E7蛋白（iE7），其含量范围为0.6至17.7 ng iE7/mg细胞蛋白。iE7在22摄氏度时不稳定（半衰期=37分钟），但在4摄氏度时相对更稳定（半衰期=210分钟）。在13例宫颈细胞学异常女性的涂片样本中，有5例检测到HPV16 E7蛋白，其浓度范围为0.10至0.69 ng iE7/mg细胞蛋白。E7蛋白检测可能在检测具有恶性潜能的HPV诱导的宫颈病变中发挥作用。