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将重酶解肌球蛋白的亚片段-1分离成两个等摩尔组分，其中一个形成反应性酶-磷酸-ADP复合物，另一个不形成。

Separation of subfragment-1 of H-meromyosin into two equimolar fractions with and without formation of the reactive enzyme-phosphate-ADP complex.

作者信息

Inoue A, Tonomura Y

出版信息

J Biochem. 1976 Feb;79(2):419-34. doi: 10.1093/oxfordjournals.jbchem.a131085.

DOI:10.1093/oxfordjournals.jbchem.a131085
PMID:131797
Abstract

H-Meromyosin (HMM) was digested with insoluble papain [EC 3.4.22.2]. Neither the size of the initial burst of Pi liberation (0.5 mole/mole of myosin head) nor the Mg2+-ATPase [EC 3.6.1.3] activity of HMM in the steady state was affected by this treatment. Acto-S-1 was obtained by mixing F-actin with HMM digested with insoluble papain (HMM-S-1). The size of the initial burst of Pi liberation of acto-S-1 was 0.35 mole/mole of S-l at an ATP concentration of 0.5 mole/mole of S-1, and 0.5 mole/moleof S-1 at ATP concentrations above 1 mole/mole of S-1...

摘要

重酶解肌球蛋白（HMM）用不溶性木瓜蛋白酶[EC 3.4.22.2]进行消化。这种处理既不影响磷酸根离子（Pi）释放初始脉冲的大小（每摩尔肌球蛋白头部释放0.5摩尔Pi），也不影响稳态下HMM的镁离子-ATP酶[EC 3.6.1.3]活性。通过将丝状肌动蛋白（F-actin）与用不溶性木瓜蛋白酶消化的HMM（HMM-S-1）混合获得肌动蛋白-S-1（Acto-S-1）。在ATP浓度为每摩尔S-1 0.5摩尔时，Acto-S-1的Pi释放初始脉冲大小为每摩尔S-1亚基0.35摩尔，而在ATP浓度高于每摩尔S-1 1摩尔时为每摩尔S-1亚基0.5摩尔……

相似文献

1
Separation of subfragment-1 of H-meromyosin into two equimolar fractions with and without formation of the reactive enzyme-phosphate-ADP complex.将重酶解肌球蛋白的亚片段-1分离成两个等摩尔组分，其中一个形成反应性酶-磷酸-ADP复合物，另一个不形成。
J Biochem. 1976 Feb;79(2):419-34. doi: 10.1093/oxfordjournals.jbchem.a131085.
2
Structure and function of the two heads of the myosin molecule. II. Separation of the two fractions of subfragment-1 of myosin by affinity column chromatography on immobilized F-actin: direct evidence for acceleration by F-actin of the decomposition of the reactive enzyme-phosphate-ADP complex formed on head B of myosin.
J Biochem. 1976 Dec;80(6):1359-69. doi: 10.1093/oxfordjournals.jbchem.a131409.
3
Structure and function of the two heads of the myosin molecule. III. Cooperativity of the two heads of the myosin molecule, shown by the effect of modification of head A with rho-chloromercuribenzoate on the interaction of head B with F-actin.肌球蛋白分子两个头部的结构与功能。III. 肌球蛋白分子两个头部的协同性，由用ρ-氯汞苯甲酸修饰头部A对头部B与F-肌动蛋白相互作用的影响所表明。
J Biochem. 1976 Dec;80(6):1371-80. doi: 10.1093/oxfordjournals.jbchem.a131410.
4
The function of two heads of myosin in muscle contraction.肌球蛋白双头在肌肉收缩中的作用。
Adv Exp Med Biol. 1988;226:227-35.
5
The amounts of adenosine di- and triphosphates bound to H-meromyosin and the adenosinetriphosphatase activity of the H-meromyosin-F-actin-relaxing protein system in the presence and absence of calcium ions. The physiological functions of the two routes of myosin adenosinetriphosphatase in muscle contraction.与重酶解肌球蛋白结合的二磷酸腺苷和三磷酸腺苷的量，以及在有钙离子和无钙离子存在的情况下，重酶解肌球蛋白 - F - 肌动蛋白 - 舒张蛋白系统的三磷酸腺苷酶活性。肌球蛋白三磷酸腺苷酶的两条途径在肌肉收缩中的生理功能。
J Biochem. 1975 Jul;78(1):83-92.
6
Reaction mechanism of Mn2+-ATPase of acto-H-meromyosin in 0.1 M KCl at 5 degrees C: evidence for the Lymn-Taylor mechanism.肌动蛋白-H-肌球蛋白的Mn2+-ATP酶在5℃下0.1M KCl中的反应机制：Lymn-Taylor机制的证据。
J Biochem. 1980 Dec;88(6):1653-62. doi: 10.1093/oxfordjournals.jbchem.a133141.
7
Desensitization of substrate inhibition of acto-H-meromyosin ATPase by treatment of H-meromyosin with rho-chloromercuribenzoate. Relation between the extent of desensitization and the amount of bound rho-chloromercuribenzoate1.用ρ-氯汞苯甲酸处理H-肌球蛋白使肌动蛋白-H-肌球蛋白ATP酶的底物抑制脱敏。脱敏程度与结合的ρ-氯汞苯甲酸量之间的关系1
J Biochem. 1975 Mar;77(3):543-57. doi: 10.1093/oxfordjournals.jbchem.a130755.
8
Mechanism of the Mg2+- and Mn2+-ATPase reactions of acto-H-meromyosin and acto-subfragment-1 in the absence of KCl at room temperature: direct decomposition of the complex of myosin-P-ADP with f-actin.室温下在无氯化钾条件下肌动蛋白-H-肌球蛋白和肌动蛋白-亚片段1的Mg2+-和Mn2+-ATP酶反应机制：肌球蛋白-P-ADP与f-肌动蛋白复合物的直接分解
J Biochem. 1980 Dec;88(6):1663-77. doi: 10.1093/oxfordjournals.jbchem.a133142.
9
Structure and function of the two heads of the myosin molecule. IV. Physiological functions of various reaction intermediates in myosin adenosinetriphosphatase, studied by the interaction between actomyosin and 8-bromoadenosine triphosphate.肌球蛋白分子两个头部的结构与功能。IV. 通过肌动球蛋白与8-溴三磷酸腺苷之间的相互作用研究肌球蛋白三磷酸腺苷酶中各种反应中间体的生理功能。
J Biochem. 1976 Dec;80(6):1381-92. doi: 10.1093/oxfordjournals.jbchem.a131411.
10
Separation of myosin subfragment 1 into two fractions, one having the burst site and the other having the non-burst site.
J Biochem. 1976 Oct;80(4):853-60. doi: 10.1093/oxfordjournals.jbchem.a131347.

引用本文的文献

1
Tryptic digestion as a probe of myosin S-1 conformation.胰蛋白酶消化作为肌球蛋白 S-1 构象的一种探测手段。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):958-62. doi: 10.1073/pnas.79.4.958.
2
The effect of pyrophosphate on the reaction of myosin with 2,4,6-trinitrobenzene sulphonate.
J Muscle Res Cell Motil. 1988 Apr;9(2):132-46. doi: 10.1007/BF01773735.
3
The role of actin in temperature-dependent gel-sol transformation of extracts of Ehrlich ascites tumor cells.肌动蛋白在艾氏腹水瘤细胞提取物温度依赖性凝胶-溶胶转变中的作用。
J Cell Biol. 1979 Feb;80(2):465-80. doi: 10.1083/jcb.80.2.465.