Evgen'ev M B, Corces V G, Lankenau D H
Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
J Mol Biol. 1992 Jun 5;225(3):917-24. doi: 10.1016/0022-2836(92)90412-d.
We have determined the DNA structure of the Ulysses transposable element of Drosophila virilis and found that this transposon is 10,653 bp and is flanked by two unusually large direct repeats 2136 bp long. Ulysses shows the characteristic organization of LTR-containing retrotransposons, with matrix and capsid protein domains encoded in the first open reading frame. In addition, Ulysses contains protease, reverse transcriptase, RNase H and integrase domains encoded in the second open reading frame. Ulysses lacks a third open reading frame present in some retrotransposons that could encode an env-like protein. A dendrogram analysis based on multiple alignments of the protease, reverse transcriptase, RNase H, integrase and tRNA primer binding site of all known Drosophila LTR-containing retrotransposon sequences establishes a phylogenetic relationship of Ulysses to other retrotransposons and suggests that Ulysses belongs to a new family of this type of elements.
我们已经确定了果蝇Ulysses转座元件的DNA结构，发现这个转座子长度为10,653碱基对，两侧是两个异常大的2136碱基对长的正向重复序列。Ulysses呈现出含长末端重复序列（LTR）反转录转座子的典型结构，其基质和衣壳蛋白结构域由第一个开放阅读框编码。此外，Ulysses在第二个开放阅读框中包含蛋白酶、逆转录酶、核糖核酸酶H和整合酶结构域。Ulysses缺少一些反转录转座子中存在的第三个开放阅读框，该开放阅读框可编码类似env的蛋白。基于所有已知果蝇含LTR反转录转座子序列的蛋白酶、逆转录酶、核糖核酸酶H、整合酶和tRNA引物结合位点的多序列比对进行的系统发育树分析，确定了Ulysses与其他反转录转座子的系统发育关系，并表明Ulysses属于这类元件的一个新家族。
