Han R N, Liu J, Tanswell A K, Post M
Department of Paediatrics, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.
Pediatr Res. 1992 May;31(5):435-40. doi: 10.1203/00006450-199205000-00004.
To study the role of basic fibroblast growth factor (bFGF) in fetal lung development, the distribution of bFGF peptide and FGF receptor (FGF-R) was examined by immunohistochemistry in embryonic and fetal rat lung [d 12 to term (term = 22 d)]. Throughout development bFGF was localized to airway epithelial cells, their basement membranes, and their extracellular matrix. FGF-R was also detected in airway epithelial cells, especially in the branching areas, and in interstitial cells as early as d 13. The number of FGF-R immunoreactive cells increased during the embryonic and pseudoglandular stages of lung development, followed by fluctuations in reactivity during the canalicular stage. No FGF-R was detected in tissue from the saccular stage of lung development. The presence of bFGF and FGF-R in developing airway epithelium and mesenchyme is compatible with a role for this growth factor during fetal lung development. In the developing lung, bFGF seems to be sequestered and stored in the extracellular matrix, and may be released at times of need. Furthermore, FGF-R up- and down-regulation offers another mechanism by which the growth of specific cell populations may be controlled during fetal lung development.
为研究碱性成纤维细胞生长因子（bFGF）在胎肺发育中的作用，采用免疫组织化学方法检测了胚胎期及胎鼠肺脏[第12天至足月（足月=22天）]中bFGF肽和FGF受体（FGF-R）的分布。在整个发育过程中，bFGF定位于气道上皮细胞、其基底膜及其细胞外基质。早在第13天，在气道上皮细胞，尤其是分支区域的上皮细胞以及间质细胞中也检测到了FGF-R。在肺发育的胚胎期和假腺期，FGF-R免疫反应性细胞数量增加，随后在小管期反应性出现波动。在肺发育的囊状期组织中未检测到FGF-R。发育中的气道上皮和间充质中存在bFGF和FGF-R，这与该生长因子在胎肺发育过程中的作用相符。在发育中的肺脏中，bFGF似乎被隔离并储存于细胞外基质中，可能在需要时释放。此外，FGF-R的上调和下调提供了另一种机制，通过该机制在胎肺发育过程中特定细胞群的生长可能受到控制。
