Guido M C, Zamorano R, Garrido-Guerrero E, Gariglio P, García-Carrancá A
Departamento de Biología Molecular, UNAM, México D.F., Mexico.
J Gen Virol. 1992 Jun;73 ( Pt 6):1395-400. doi: 10.1099/0022-1317-73-6-1395.
The physical state of the human papillomavirus (HPV) genome is usually different in malignant lesions of the skin, in which it is generally found in episomal form, and genital mucosa, in which it is frequently integrated with disruption of the E2 gene. Using chimeric or natural HPV promoters in the presence of the bovine papillomavirus type 1 E2 gene product, we observed transcription activation or repression, depending on the distance of E2-binding motifs from the start site. We found a clear difference in the positions of E2-binding motifs in cutaneous and genital HPVs that may partly explain the selective pressure for genome integration of genital HPV types in malignant lesions.
人乳头瘤病毒（HPV）基因组的物理状态在皮肤恶性病变和生殖器黏膜中通常有所不同，在皮肤恶性病变中，HPV基因组一般以游离型存在，而在生殖器黏膜中，它常与E2基因的破坏整合在一起。在存在1型牛乳头瘤病毒E2基因产物的情况下，使用嵌合或天然HPV启动子，我们观察到转录激活或抑制，这取决于E2结合基序与起始位点的距离。我们发现皮肤型和生殖器型HPV中E2结合基序的位置存在明显差异，这可能部分解释了生殖器型HPV在恶性病变中基因组整合的选择压力。
