使用生物素化DNA探针通过原位杂交检测宫颈病变中的人乳头瘤病毒DNA序列。

Detection of human papillomavirus DNA sequences in cervical lesions by in situ hybridization using biotinylated DNA probes.

作者信息

Zhang Z S, Wu Y L, Xu L L, Yang Y H

机构信息

Department of Obstetrics and Gynecology, Second Affiliated Hospital, Hunan Medical University, Changsha.

出版信息

Chin Med J (Engl). 1992 Apr;105(4):293-7.

PMID:1319885

Abstract

Ninety-six biopsy specimens were collected for studying the association of specific sequences of HPV DNA with genital warts, precancerous lesions and invasive cervical cancer. Using DNA probes labeled with biotin, 17 of the 22 (77.27%) specimens showing condyloma and 1 of the 14 (7.14%) specimens displaying subclinical papillomavirus infection (SPI) of cervix were found to be positive for HPV 6/11; 4 of 23 (17.39%) specimens of squamous epithelial cervical cancer and 2 of the 23 (8.70%) specimens of cervical intraepithelial neoplasia (CIN) were positive for HPV 16/18. All the 14 specimens of cervicitis were negative for both probes. The HPV 6/11 positive hybridization products were present mainly in the superficial and intermediate epithelium of condyloma and SPI. In invasive cancer, HPV 16/18 were shown more often in cancer cells of infiltrative nests and were focally distributed.

摘要

收集了96份活检标本，用于研究人乳头瘤病毒（HPV）DNA的特定序列与尖锐湿疣、癌前病变及浸润性宫颈癌之间的关联。使用生物素标记的DNA探针，在22份显示尖锐湿疣的标本中有17份（77.27%）、在14份显示宫颈亚临床乳头瘤病毒感染（SPI）的标本中有1份（7.14%）检测出HPV 6/11呈阳性；在23份宫颈鳞状上皮癌标本中有4份（17.39%）、在23份宫颈上皮内瘤变（CIN）标本中有2份（8.70%）检测出HPV 16/18呈阳性。所有14份宫颈炎标本两种探针检测均为阴性。HPV 6/11阳性杂交产物主要存在于尖锐湿疣和SPI的表层及中层上皮。在浸润性癌中，HPV 16/18在浸润巢的癌细胞中更常见，且呈局灶性分布。