Zhang W, Sun Y, Shang M, Jin S, Liang X, Liang K, Wu A, Nan G, Wang X, Zhang W
Cancer Institute, CAMS, Beijing.
Proc Chin Acad Med Sci Peking Union Med Coll. 1990;5(3):174-5.
Thirty-eight cervical carcinomas and 1 cervical condyloma were examined for the presence of human papillomavirus (HPV) type 16 DNA using 3H-dCTP-labelled HPV-16 DNA probe in paraffin section in situ hybridization and 32P-dCTP-labelled probe in dot blot hybridization (Tm-17). The results showed an HPV-16 DNA positive rate in our series of about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. There was no significant difference between the two methods. We thus confirmed that paraffin section in situ hybridization is an informative, reliable, and sensitive method for the diagnosis of cervical HPV infection.
采用3H-dCTP标记的HPV-16 DNA探针,通过石蜡切片原位杂交法,以及32P-dCTP标记的探针,通过斑点印迹杂交法(Tm-17),对38例宫颈癌和1例宫颈湿疣进行人乳头瘤病毒16型(HPV-16)DNA检测。结果显示,通过石蜡切片原位杂交法,我们这组病例中HPV-16 DNA阳性率约为74.4%(29/39),通过斑点印迹杂交法阳性率约为71.8%(28/29)。两种方法之间无显著差异。因此,我们证实石蜡切片原位杂交是诊断宫颈HPV感染的一种信息丰富、可靠且敏感的方法。