γ-氨基丁酸A型（GABAA）拮抗剂揭示了小脑颗粒细胞层中[35S]叔丁基二环磷硫酰胺（TBPS）的结合位点。
GABAA antagonists reveal binding sites for [35S]TBPS in cerebellar granular cell layer.
作者信息
Korpi E R, Lüddens H, Seeburg P H
机构信息
Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, F.R.G.
出版信息
Eur J Pharmacol. 1992 Feb 18;211(3):427-8. doi: 10.1016/0014-2999(92)90402-p.
Autoradiography revealed picrotoxin-sensitive [35S]TBPS ([35S]t-butylbicyclophosphorothionate) binding sites in the cerebellar granule cell layer only in the presence of GABAA receptor antagonists. However, under the same conditions, the binding was decreased in other brain regions, including the cerebellar molecular cell layer. Thus, the convulsant binding sites at GABAA receptors in the granule cell layer are highly sensitive to down-regulation by GABA, which may be due to a unique combination of receptor subunits.
放射自显影显示，仅在存在γ-氨基丁酸A型（GABAA）受体拮抗剂的情况下，在小脑颗粒细胞层中存在对荷包牡丹碱敏感的[35S]叔丁基双环磷硫代酸酯（[35S]TBPS）结合位点。然而，在相同条件下，包括小脑分子细胞层在内的其他脑区的结合减少。因此，颗粒细胞层中GABAA受体处的惊厥性结合位点对γ-氨基丁酸（GABA）介导的下调高度敏感，这可能是由于受体亚基的独特组合所致。
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