On N T, Damani L A, Rance D J
Chelsea Department of Pharmacy, King's College London, UK.
J Chromatogr. 1992 Feb 14;574(2):352-5. doi: 10.1016/0378-4347(92)80052-r.
A method is described for the determination of UK-57,400 (I), a 6-substituted quinoline cardiotonic, and its pyridyl-N-oxide in dog plasma. The analytes are selectively retained from plasma (1 ml) on a solid-phase extraction column and eluted with 1 ml of methanol. After evaporation to dryness, the residue is reconstituted in 100 microliters of the mobile phase. Chromatography is carried out on a Spherisorb 5 microns phenyl high-performance liquid chromatography column, with ultraviolet detection. Calibration curves are linear for concentrations from 10 to 100 ng ml-1. For I, the coefficients of variation at highest and lowest concentrations are 1 and 14%, respectively, while the corresponding figures for the pyridyl-N-oxide metabolite are 4 and 10%, respectively. Sample recovery from extraction is greater than 90%. The limit of detection is 4 ng ml-1 for both analytes.
本文描述了一种测定犬血浆中6-取代喹啉强心剂UK-57,400（I）及其吡啶-N-氧化物的方法。分析物从1毫升血浆中在固相萃取柱上被选择性保留，并用1毫升甲醇洗脱。蒸发至干后，残渣用100微升流动相复溶。在Spherisorb 5微米苯基高效液相色谱柱上进行色谱分析，采用紫外检测。校准曲线在10至100纳克/毫升的浓度范围内呈线性。对于I，最高和最低浓度下的变异系数分别为1%和14%，而吡啶-N-氧化物代谢物的相应数字分别为4%和10%。萃取的样品回收率大于90%。两种分析物的检测限均为4纳克/毫升。
