源自多形性胶质母细胞瘤的两种细胞系的建立与鉴定
Establishment and characterization of two cell lines derived from human glioblastoma multiforme.
作者信息
Bacciocchi G, Gibelli N, Zibera C, Pedrazzoli P, Bergamaschi G, De Piceis Polver P, Danova M, Mazzini G, Palomba L, Tupler R
机构信息
Divisione di Oncologia, IRCCS Fondazione Clinica del Lavoro, Pavia, Italy.
出版信息
Anticancer Res. 1992 May-Jun;12(3):853-61.
We established and characterized two cell lines derived from glioblastoma multiforme. Both cell lines exhibited tumor cell morphology and growth kinetics and showed variable expression of glial fibrillary acidic protein (GFAP), S-100, fibronectin and vimentin. Cytofluorimetrical analysis of tumor samples showed a diploid DNA distribution, whereas permanent culture cells evolved to the hyperdiploid DNA content. Karyotype studies revealed cytogenetical abnormalities described in glial tumors including gain of chromosome 7, loss of chromosome 10 and presence of double minutes (DMs). Enhanced expression of Ha-ras and c-myc genes resulted from high p-21 and p-62 levels. The contemporary presence of TGF-alpha and EGF-Rc transcripts suggested an autocrine mechanism in the cell lines growth.
我们建立并鉴定了源自多形性胶质母细胞瘤的两种细胞系。两种细胞系均呈现肿瘤细胞形态和生长动力学，并显示出胶质纤维酸性蛋白（GFAP）、S-100、纤连蛋白和波形蛋白的表达存在差异。肿瘤样本的细胞荧光分析显示为二倍体DNA分布，而永久培养细胞则演变为超二倍体DNA含量。核型研究揭示了胶质肿瘤中描述的细胞遗传学异常，包括7号染色体增加、10号染色体缺失和双微体（DMs）的存在。高p-21和p-62水平导致Ha-ras和c-myc基因表达增强。TGF-α和EGF-Rc转录本的同时存在提示了细胞系生长中的自分泌机制。
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