Morgan T R, Morgan K, Vaziri N D, Pandian M R
Department of Medicine, VA Medical Center, Long Beach, California 90822.
Life Sci. 1992;51(5):337-44. doi: 10.1016/0024-3205(92)90585-d.
Atrial natriuretic peptide (ANP) is a cardiac hormone with natriuretic and diuretic effects. To better define the ANP hormonal system in the nephrotic syndrome, a condition associated with renal sodium retention, we undertook a study of glomerular ANP receptors in rats with puromycin aminonucleoside-induced nephrotic syndrome and in pair-fed controls. Nephrotic rats had significantly decreased serum albumin and total protein and significantly increased serum cholesterol, triglycerides and 24 hour urinary protein excretion. Plasma level of atrial natriuretic peptide was similar in both groups of rats. Competition binding inhibition studies in isolated glomeruli demonstrated one binding site in both groups of rats. The density of ANP binding sites in isolated glomeruli was similar in nephrotic and pair-fed rats while the binding affinity was increased significantly in the nephrotic rats. This is the first study to demonstrate alterations in renal ANP receptors in the nephrotic syndrome. Further studies will be necessary to determine whether alterations in glomerular ANP receptors contribute to renal sodium retention in the nephrotic syndrome.
心房利钠肽（ANP）是一种具有利钠和利尿作用的心脏激素。为了更好地界定肾病综合征（一种与肾脏钠潴留相关的病症）中的ANP激素系统，我们对嘌呤霉素氨基核苷诱导的肾病综合征大鼠及配对饲养的对照大鼠的肾小球ANP受体进行了研究。肾病大鼠的血清白蛋白和总蛋白显著降低，血清胆固醇、甘油三酯和24小时尿蛋白排泄显著增加。两组大鼠的心房利钠肽血浆水平相似。对分离肾小球进行的竞争结合抑制研究表明，两组大鼠均有一个结合位点。肾病大鼠和配对饲养大鼠分离肾小球中ANP结合位点的密度相似，而肾病大鼠的结合亲和力显著增加。这是第一项证明肾病综合征中肾脏ANP受体发生改变的研究。有必要进一步开展研究，以确定肾小球ANP受体的改变是否导致肾病综合征中的肾脏钠潴留。
