Knight A I, Ni H, Cartwright K A, McFadden J J
School of Biological Sciences, University of Surrey, Guildford, UK.
Mol Microbiol. 1992 Jun;6(11):1565-73. doi: 10.1111/j.1365-2958.1992.tb00878.x.
Examination of Neisseria meningitidis strains associated with endemic meningococcal disease demonstrated differences in the number of copies of a repetitive sequence. Characterization of a copy of this repetitive sequence present in B15 strains has revealed the presence of a novel insertion sequence (IS1106) located within a complex repetitive region downstream of the gene for the major surface antigen (porA). IS1106 has a length of 1137 bp and is flanked by 36bp inverted repeats. Two open reading frames (ORF1 and ORF2) are present in opposite strands in codon-codon register with ORF2 entirely located within ORF1. The predicted protein from ORF1 demonstrates homology with the 5A protein of IS5 (Kroger and Hobom, 1982). Strains from two independent outbreaks of B15 meningococcal disease in the UK were found to contain the same genomic deletion removing a copy of IS1106 downstream of the porA gene.
对与地方性脑膜炎球菌病相关的脑膜炎奈瑟菌菌株进行检测，结果显示一个重复序列的拷贝数存在差异。对B15菌株中存在的该重复序列的一个拷贝进行特征分析，发现一个新的插入序列（IS1106）存在于主要表面抗原（porA）基因下游的一个复杂重复区域内。IS1106长度为1137 bp，两侧为36 bp的反向重复序列。两个开放阅读框（ORF1和ORF2）位于相反的链上，密码子对应排列，其中ORF2完全位于ORF1内。从ORF1预测的蛋白质与IS5的5A蛋白具有同源性（克罗格和霍博姆，1982年）。在英国两次独立的B15脑膜炎球菌病暴发中分离出的菌株，被发现含有相同的基因组缺失，该缺失去除了porA基因下游的一个IS1106拷贝。
