Mailleux P, Takazawa K, Albala N, Erneux C, Vanderhaeghen J J
Laboratory of Neuropathology and Neuropeptide Research, Université Libre de Bruxelles, Brussels, Belgium.
Neurosci Lett. 1992 Mar 16;137(1):69-71. doi: 10.1016/0304-3940(92)90300-v.
The distribution of the messenger RNA coding for the recently cloned inositol 1,4,5-trisphosphate (InsP3) 3-kinase, the enzyme phosphorylating InsP3 to InsP4, was compared to the localizations of InsP3 receptor mRNA in the human brain using in situ hybridization histochemistry and oligonucleotide probes. InsP3 3-kinase and receptor mRNA levels were high in the cerebellar Purkinje cells. They were also observed, to a much lesser degree than in the cerebellum, in the hippocampal CA1 pyramidal cells and dentate gyrus granule cells, in the majority of the cortical neurons and in the striatal medium-sized neurons. Both mRNAs were not detected in the brainstem and in the glial cells.
利用原位杂交组织化学和寡核苷酸探针，将编码最近克隆的肌醇1,4,5-三磷酸（InsP3）3-激酶（将InsP3磷酸化为InsP4的酶）的信使核糖核酸的分布，与人脑InsP3受体信使核糖核酸的定位进行了比较。InsP3 3-激酶和受体信使核糖核酸水平在小脑浦肯野细胞中较高。在海马CA1锥体细胞和齿状回颗粒细胞、大多数皮质神经元以及纹状体中型神经元中也观察到了它们，但程度远低于小脑。在脑干和神经胶质细胞中未检测到这两种信使核糖核酸。
