糖尿病红细胞中的磷脂N-甲基化：对膜钠钾ATP酶活性的影响。

Phospholipid N-methylation in diabetic erythrocytes: effects on membrane Na+, K+ ATPase activity.

作者信息

Kowluru A, Kowluru R A

机构信息

Division of Life Sciences, Los Alamos National Laboratory, University of California, NM 87545.

出版信息

Cell Biochem Funct. 1992 Jun;10(2):95-101. doi: 10.1002/cbf.290100205.

DOI:10.1002/cbf.290100205

PMID:1321009

Abstract

Phospholipid methylation was quantified in non-diabetic and streptozotocin diabetic rat erythrocytes. While the total mass of methylated lipids remained the same in both groups, the relative abundance of individual methylated lipid species differed significantly in diabetic erythrocytes. Moreover, incubation of erythrocytes membranes with S-adenosyl methionine, a substrate for methyl transferases, not only increased membrane lipid methylation but also decreased Na+, K+ ATPase activity significantly. These results suggest that phospholipid methylation may cause the observed depression of erythrocyte Na+, K+ ATPase activity in diabetes and could contribute to the altered rheology of erythrocytes in diabetes.

摘要

在非糖尿病和链脲佐菌素诱导的糖尿病大鼠红细胞中对磷脂甲基化进行了定量分析。虽然两组中甲基化脂质的总质量保持不变，但糖尿病红细胞中各个甲基化脂质种类的相对丰度存在显著差异。此外，用甲基转移酶的底物S-腺苷甲硫氨酸孵育红细胞膜，不仅增加了膜脂质甲基化，还显著降低了Na +、K + -ATP酶活性。这些结果表明，磷脂甲基化可能导致糖尿病中观察到的红细胞Na +、K + -ATP酶活性降低，并可能导致糖尿病中红细胞流变学改变。