Pari L, Saravanan R
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamilnadu 608002, India.
Chem Biol Interact. 2007 Aug 15;169(1):15-24. doi: 10.1016/j.cbi.2007.04.010. Epub 2007 May 4.
Succinic acid monoethyl ester (EMS) was recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. In the present study the effect of EMS and metformin on erythrocyte membrane bound enzymes and antioxidants activity in plasma and erythrocytes of streptozotocin-nicotinamide induced type 2 diabeteic model was investigated. Succinic acid monoethyl ester was administered intraperitonially for 30 days to control and diabetic rats. The effect of EMS on glucose, insulin, hemoglobin, glycosylated hemoglobin, TBARS, hydroperoxide, superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (Gpx), glutathione-S-transferase (GST), vitamins C and E, reduced glutathione (GSH) and membrane bound enzymes were studied. The effect of EMS was compared with metformin, a reference drug. The levels of glucose, glycosylated hemoglobin, TBARS, hyderoperoxide, and vitamin E were increased significantly whereas the level of insulin and hemoglobin, as well as antioxidants (SOD, CAT, Gpx, GST, vitamin C and GSH) membrane bound total ATPase, Na(+)/K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase were decreased significantly in streptozotocin-nicotinamide diabetic rats. Administration of EMS to diabetic rats showed a decrease in the levels of glucose, glycosylated hemoglobin, lipid peroxidation markers and vitamin E. In addition the levels of insulin, hemoglobin, enzymic antioxidants, vitamin C, and GSH and the activities of membrane bound enzymes also were increased in EMS and metformin treated diabetic rats. The present study indicates that the EMS possesses a significant beneficial effect on erythrocyte membrane bound enzymes and antioxidants defense system in addition to its antidiabetic effect.
琥珀酸单乙酯（EMS）最近被提议作为一种促胰岛素分泌剂用于治疗非胰岛素依赖型糖尿病。在本研究中，研究了EMS和二甲双胍对链脲佐菌素-烟酰胺诱导的2型糖尿病模型大鼠血浆和红细胞中红细胞膜结合酶以及抗氧化剂活性的影响。将琥珀酸单乙酯腹腔注射给正常大鼠和糖尿病大鼠30天。研究了EMS对血糖、胰岛素、血红蛋白、糖化血红蛋白、硫代巴比妥酸反应物（TBARS）、氢过氧化物、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（Gpx）、谷胱甘肽-S-转移酶（GST）、维生素C和E、还原型谷胱甘肽（GSH）以及膜结合酶的影响。将EMS的作用与参考药物二甲双胍进行了比较。在链脲佐菌素-烟酰胺糖尿病大鼠中，血糖、糖化血红蛋白、TBARS、氢过氧化物和维生素E水平显著升高，而胰岛素和血红蛋白水平以及抗氧化剂（SOD、CAT、Gpx、GST、维生素C和GSH）、膜结合总ATP酶、钠/钾-ATP酶、钙-ATP酶和镁-ATP酶活性显著降低。给糖尿病大鼠施用EMS后，血糖、糖化血红蛋白、脂质过氧化标志物和维生素E水平降低。此外在接受EMS和二甲双胍治疗的糖尿病大鼠中，胰岛素、血红蛋白、酶促抗氧化剂、维生素C和GSH水平以及膜结合酶活性也有所增加。本研究表明，EMS除了具有抗糖尿病作用外，对红细胞膜结合酶和抗氧化防御系统也具有显著的有益作用。
