Groene W S, Shaw R D
Department of Medicine/Gastroenterology, Northport Veterans Administration Medical Center, NY 11768-2290.
J Virol Methods. 1992 Jul;38(1):93-102. doi: 10.1016/0166-0934(92)90172-a.
The use of the synthetic psoralen 4'-aminomethyl-4,5',8-trimethylpsoralen hydrochloride (AMT) is described for the inactivation of infectious rotavirus, a member of the viral family Reoviradae with a double-stranded RNA genome. This method not only provides complete inactivation of the virus but leaves antigenically intact particles. The lack of viral replication following inactivation was determined with an immunohistochemical focus assay. The antigenic authenticity of the particles was determined by monoclonal antibody ELISA and a viral hemagglutination assay.
描述了合成补骨脂素4'-氨甲基-4,5',8-三甲基补骨脂素盐酸盐（AMT）用于灭活传染性轮状病毒的用途，轮状病毒是呼肠孤病毒科的成员，具有双链RNA基因组。该方法不仅能完全灭活病毒，还能使颗粒在抗原性上保持完整。通过免疫组织化学焦点测定法确定灭活后病毒是否缺乏复制能力。通过单克隆抗体ELISA和病毒血凝试验确定颗粒的抗原真实性。
