Dicou E
INSERM U 298, CHRU, Angers, France.
Brain Res Mol Brain Res. 1992 Jun;14(1-2):136-8. doi: 10.1016/0169-328x(92)90021-3.
A nerve growth factor (NGF) precursor form of about 24 kDa was identified in homogenates of rat thyroid and hippocampus by immunoprecipitation using three sera raised against a synthetic peptide that reproduces the sequence -71 to -46 of the proNGF molecule. Besides this species, a 31 kDa protein, as well as a cleavage product of 12 kDa were also immunoprecipitated in both tissues by one of these sera.
通过使用针对一种合成肽产生的三种血清进行免疫沉淀，在大鼠甲状腺和海马体的匀浆中鉴定出一种约24 kDa的神经生长因子（NGF）前体形式，该合成肽再现了proNGF分子的-71至-46序列。除了这种形式外，这三种血清中的一种还在两种组织中免疫沉淀出一种31 kDa的蛋白质以及一种12 kDa的裂解产物。
