盐皮质激素在植物细胞中的作用。

Mineralocorticoid hormone action in plant cells.

作者信息

Mirshahi M, Mirshahi A, Nato A, Agarwal M K

机构信息

INSERM U-86, Centre Universitaire des Cordeliers, Paris, France.

出版信息

Biochem Biophys Res Commun. 1992 Jul 31;186(2):1102-7. doi: 10.1016/0006-291x(92)90860-n.

DOI:10.1016/0006-291x(92)90860-n

PMID:1323283

Abstract

The multiplication of Chlamydomonas reinhardtii wild type cells can be arrested by the spirolactone RU 26752 and this is fully reversible by the natural mineralocorticoid aldosterone. Evidence is presented for a 52 kDa protein that possesses functional DNA and ligand binding domains and tests positive for mineralocorticoid receptor-like activity by immuneprecipitation, macroaggregation, and photoaffinity. The regulation of trans-activation by steroid hormones in the animal world would therefore appear to be just as valid for the plant kingdom, thereby providing a new model for genetic analysis.

摘要

莱茵衣藻野生型细胞的增殖可被螺内酯RU 26752阻断，而天然盐皮质激素醛固酮可使其完全恢复。有证据表明存在一种52 kDa的蛋白质，它具有功能性DNA和配体结合结构域，并通过免疫沉淀、宏观聚集和光亲和性检测出具有盐皮质激素受体样活性呈阳性。因此，在动物界中类固醇激素对反式激活的调节在植物界似乎同样有效，从而为遗传分析提供了一个新模型。