Gotoh N, Tojo A, Hino M, Yazaki Y, Shibuya M
Department of Genetics, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1992 Jul 31;186(2):768-74. doi: 10.1016/0006-291x(92)90812-y.
Epidermal growth factor receptor (EGF-R) is a widely expressed ligand-dependent tyrosine kinase. The tyrosine residue at 845 in EGF-R corresponds to Y416 of v/c-src kinase, which is highly conserved and functionally important in many tyrosine kinases. To clarify the functional role of Y845, we constructed a mutant human EGF-R in which this tyrosine was replaced with phenylalanine and transfected it to NIH3T3 cells. EGF-R F845 induced EGF-dependent cellular transformation and revealed tyrosine-autophosphorylation of a 170 kDa protein, and initiated DNA synthesis similar to the wild-type EGF-R. We conclude here that Y845 is dispensable in the above mentioned functions of EGF-R tyrosine kinase.
表皮生长因子受体（EGF-R）是一种广泛表达的依赖配体的酪氨酸激酶。EGF-R中845位的酪氨酸残基对应于v/c-src激酶的Y416，在许多酪氨酸激酶中高度保守且功能重要。为阐明Y845的功能作用，我们构建了一个将该酪氨酸替换为苯丙氨酸的突变型人EGF-R，并将其转染至NIH3T3细胞。EGF-R F845诱导了依赖EGF的细胞转化，显示出170 kDa蛋白的酪氨酸自磷酸化，并启动了与野生型EGF-R相似的DNA合成。我们在此得出结论，Y845在EGF-R酪氨酸激酶的上述功能中是不必要的。
