花生四烯酸及其辅酶A酯对兔肾皮质中前列腺素E2分解代谢的影响。
The effects of arachidonic acid and its CoA ester on the catabolism of prostaglandin E2 in rabbit kidney cortex.
作者信息
Sakuma S, Fujimoto Y, Okumura M, Nishida H, Yamamoto I, Fujita T
机构信息
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
出版信息
Biochem Int. 1992 Jul;27(2):291-9.
The effects of arachidonic acid and arachidonoyl CoA on the catabolism of prostaglandin E2 in a 105000 x g supernatant fraction from rabbit kidney cortex were examined. Arachidonic acid reduced the 15-hydroxy prostaglandin dehydrogenase activity by 50% at 20 microM, while arachidonoyl CoA showed weak inhibition for the enzyme activity (15% at 20 microM). However, arachidonoyl CoA strongly inhibited the prostaglandin delta 13 reductase activity, the concentration required for 50% inhibition being about 3 microM. The dehydrogenase inhibition by arachidonic acid was non-competitive with regard to NAD+ and prostaglandin E2, respectively. Arachidonoyl CoA was also a non-competitive inhibitor for the reductase with regard to NADH and 15-keto prostaglandin E2, respectively. These results suggest that arachidonic acid and arachidonoyl CoA can be important modulating factors in prostaglandin catabolism by the kidney cortex.
研究了花生四烯酸和花生四烯酰辅酶A对兔肾皮质105000×g上清液组分中前列腺素E2分解代谢的影响。花生四烯酸在20μM时使15-羟基前列腺素脱氢酶活性降低50%，而花生四烯酰辅酶A对该酶活性表现出较弱的抑制作用（20μM时为15%）。然而，花生四烯酰辅酶A强烈抑制前列腺素δ13还原酶活性，50%抑制所需浓度约为3μM。花生四烯酸对脱氢酶的抑制作用分别对NAD+和前列腺素E2而言是非竞争性的。花生四烯酰辅酶A对还原酶而言分别对NADH和15-酮基前列腺素E2也是非竞争性抑制剂。这些结果表明，花生四烯酸和花生四烯酰辅酶A可能是肾皮质前列腺素分解代谢中的重要调节因子。
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