Zenvirth D, Arbel T, Sherman A, Goldway M, Klein S, Simchen G
Department of Genetics, Hebrew University of Jerusalem, Israel.
EMBO J. 1992 Sep;11(9):3441-7. doi: 10.1002/j.1460-2075.1992.tb05423.x.
We present a scheme for locating double-strand breaks (DSBs) in meiotic chromosomes of Saccharomyces cerevisiae, based on the separation of large DNA molecules by pulsed field gel electrophoresis. Using a rad50S mutant, in which DSBs are not processed, we show that DSBs are widely induced in S. cerevisiae chromosomes during meiosis. Some of the DSBs accumulate at certain preferred sites. We present general profiles of DSBs in chromosomes III, V, VI and VII. A map of the 12 preferred sites on chromosome III is presented. At least some of these sites correlate with known 'hot spots' for meiotic recombination. The data are discussed in view of current models of meiotic recombination and chromosome segregation.
我们提出了一种基于脉冲场凝胶电泳分离大DNA分子来定位酿酒酵母减数分裂染色体中双链断裂（DSB）的方案。使用一种不处理DSB的rad50S突变体，我们发现减数分裂期间DSB在酿酒酵母染色体中广泛诱导产生。一些DSB在特定的偏好位点积累。我们展示了III、V、VI和VII号染色体中DSB的总体分布情况。给出了III号染色体上12个偏好位点的图谱。这些位点中至少有一些与已知的减数分裂重组“热点”相关。我们根据当前的减数分裂重组和染色体分离模型对这些数据进行了讨论。
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