酵母着丝粒的分离及功能性小环状染色体的构建。
Isolation of a yeast centromere and construction of functional small circular chromosomes.
作者信息
Clarke L, Carbon J
出版信息
Nature. 1980 Oct 9;287(5782):504-9. doi: 10.1038/287504a0.
DOI:10.1038/287504a0
PMID:6999364
Abstract
The centromeric DNA (CEN3) from yeast chromosome III has been isolated on a 1.6 kilobase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae. When present on a plasmid carrying a yeast chromosomal replicator, CEN3 enables that plasmid to function as a chromosome both mitotically and meiotically. Minichromosomes containing CEN3 are stable in mitosis and segregate as ordinary yeast chromosomes in the first and second meiotic divisions.
摘要
酵母三号染色体的着丝粒DNA（CEN3）已在一段1.6千碱基对的DNA片段上分离出来，该片段位于酿酒酵母着丝粒连接的CDC10基因座附近。当CEN3存在于携带酵母染色体复制子的质粒上时，它能使该质粒在有丝分裂和减数分裂过程中都发挥染色体的功能。含有CEN3的微型染色体在有丝分裂中是稳定的，并在第一次和第二次减数分裂中像普通酵母染色体一样分离。
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