Iwahashi H, Parker C E, Tomer K B, Mason R P
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Free Radic Res Commun. 1992;16(5):295-301. doi: 10.3109/10715769209049182.
HPLC-EPR analyses of the reaction mixtures of microsomal suspensions incubated with ADP, ferric chloride, NADPH and alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN) were performed. In the elution pattern of the reaction mixture, three peaks (peaks 1, 2 and 3) were detected. The radical adducts (1 and 3) were identified as being the pentyl- and ethyl-radical adducts of 4-POBN by comparing their retention times with those of the authentic radical adducts.
对微粒体悬浮液与ADP、氯化铁、NADPH和α-(4-吡啶-1-氧化物)-N-叔丁基硝酮(4-POBN)一起孵育的反应混合物进行了高效液相色谱-电子顺磁共振(HPLC-EPR)分析。在反应混合物的洗脱图谱中，检测到三个峰(峰1、峰2和峰3)。通过将自由基加合物(1和3)的保留时间与相应标准自由基加合物的保留时间进行比较，鉴定出它们分别为4-POBN的戊基和乙基自由基加合物。
