Mills J B, Zeringue M, Harris R, Wilhelmi A E, Rudman D
J Clin Endocrinol Metab. 1976 Jun;42(6):1127-32. doi: 10.1210/jcem-42-6-1127.
The possible somatotropic effect in man of porcine growth hormone (pGH) and its plasmin digest has not been comprehensively studied before. For this purpose, pGH was digested with rat or human plasmin; acrylamide gel electrophoresis showed less than 10% native pGH remaining in the digests. Native pGH and the 2 types of plasmin digest possessed similar GH potency, as measured by the weight gain assay in the hypophysectomized rat: 1-2 IU/mg. In 7 GH-deficient children and 3 adults with myotonic dystrophy, we measured the capacity of human GH (hGH), pGH, and pGH plasmin digests to cause: a) the retention of N, P, K, Na, and Cl; b) a rise in plasma free fatty acids; c) a fall in plasma alpha-amino NL d) impaired glucose tolerance; and e) hyperinsulinemia. Human GH was active in all respects at minimal effective dosages of .0168 to 0.168 IU/kg BW(3/4) per day. The pGH preparations had no detectable effect at 0.532 I.U./kg BW(3/4)/day. The data show that in man pGH and its plasmin digests possess less than 1/30, less than 1/10, and less than 1/3 the anabolic, adipokinetic, and diabetogenic potencies of hGH, respectively.
此前尚未对猪生长激素（pGH）及其纤溶酶消化产物对人体可能产生的促生长作用进行全面研究。为此，用大鼠或人纤溶酶消化pGH；丙烯酰胺凝胶电泳显示消化产物中残留的天然pGH不到10%。通过垂体切除大鼠的体重增加试验测定，天然pGH和两种纤溶酶消化产物具有相似的生长激素效价：1 - 2 IU/mg。在7名生长激素缺乏儿童和3名患有强直性肌营养不良的成年人中，我们测定了人生长激素（hGH）、pGH和pGH纤溶酶消化产物引起以下情况的能力：a）氮、磷、钾、钠和氯的潴留；b）血浆游离脂肪酸升高；c）血浆α-氨基氮降低；d）糖耐量受损；e）高胰岛素血症。人生长激素在每天0.0168至0.168 IU/kg BW(3/4)的最小有效剂量下在各方面均有活性。pGH制剂在0.532 I.U./kg BW(3/4)/天的剂量下未检测到效果。数据表明，在人体中，pGH及其纤溶酶消化产物的合成代谢、脂肪代谢和致糖尿病效力分别不到hGH的1/30、1/10和1/3。
