Clyne C D, Williams B C, Walker S W, Bird I M
University Department of Pharmacology, Edinburgh, U.K.
Biochem Pharmacol. 1992 Aug 4;44(3):441-6. doi: 10.1016/0006-2952(92)90434-k.
The effects of acetylcholine (ACh) and manganese pre-incubation on angiotensin II (AII)-stimulated incorporation of [3H]inositol into phosphoinositide, phosphoinositol and free inositol fractions of adrenocortical cells isolated from the bovine zona fasciculata/reticularis (zfr) were investigated. In cells pre-labelled for 6 hr with [3H]inositol, ACh and AII stimulated the incorporation of cytosolic [3H]inositol into a common hormone-sensitive pool of phosphoinositides, which was distinct from the non-hormone-sensitive pool labelled in the presence of manganese. Regression analysis of the cortisol versus [3H]inositol headgroup responses for both AII (10(-11)-10(-7) M) and ACh (10(-9)-10(-3) M) showed that the gradients of these responses were not significantly different. These data provide strong evidence that in cultured bovine zfr cells, ACh and AII stimulate the breakdown and resynthesis of a common pool of phosphoinositides.
研究了乙酰胆碱（ACh）和锰预孵育对从牛束状带/网状带（zfr）分离的肾上腺皮质细胞中血管紧张素II（AII）刺激的[3H]肌醇掺入磷酸肌醇、磷酸肌醇和游离肌醇部分的影响。在用[3H]肌醇预标记6小时的细胞中，ACh和AII刺激胞质[3H]肌醇掺入一个共同的激素敏感磷酸肌醇池，该池与在锰存在下标记的非激素敏感池不同。对AII（10（-11）-10（-7）M）和ACh（10（-9）-10（-3）M）的皮质醇与[3H]肌醇头部基团反应进行回归分析表明，这些反应的梯度没有显著差异。这些数据提供了强有力的证据，表明在培养的牛zfr细胞中，ACh和AII刺激共同的磷酸肌醇池的分解和再合成。
