Yamada M, Kakita A, Mizuguchi M, Rhee S G, Kim S U, Ikuta F
Department of Pathology, Niigata University, Japan.
Brain Res. 1992 Apr 24;578(1-2):41-8. doi: 10.1016/0006-8993(92)90227-z.
Subcellular localization of inositol 1,4,5-trisphosphate 3-kinase in the rat cerebellar cortex was studied immunohistochemically using a monoclonal antibody. Electron microscopy revealed intense immunoreactivity in the dendritic spines of Purkinje cells forming synapses with the parallel fibers, climbing fibers and recurrent collaterals of Purkinje cell axons. The labelling was associated with the hypolemmal cisternae, surrounding matrix and plasmalemma including the postsynaptic densities. Weaker immunoreactivity was present in the dendritic spines of basket cells and in certain segments of Purkinje cell recurrent collaterals. The postsynaptic regions of the dendritic trunks of Purkinje and basket cells were negative. These results indicate that inositol 1,4,5-trisphosphate 3-kinase is distributed amongst the spines of various synaptic relations with different electrophysiological properties, and that axon terminals of certain cell types are another functional site for the enzyme.
使用单克隆抗体通过免疫组织化学方法研究了大鼠小脑皮质中肌醇1,4,5 -三磷酸3 -激酶的亚细胞定位。电子显微镜显示，在浦肯野细胞的树突棘中存在强烈的免疫反应性，这些树突棘与平行纤维、攀缘纤维以及浦肯野细胞轴突的回返侧支形成突触。标记物与膜下池、周围基质以及包括突触后致密物在内的质膜相关。篮状细胞的树突棘和浦肯野细胞回返侧支的某些节段存在较弱的免疫反应性。浦肯野细胞和篮状细胞树突干的突触后区域呈阴性。这些结果表明，肌醇1,4,5 -三磷酸3 -激酶分布于具有不同电生理特性的各种突触关系的棘中，并且某些细胞类型的轴突终末是该酶的另一个功能位点。
