Han Y H, Smibert R M, Krieg N R
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Can J Microbiol. 1992 Feb;38(2):104-10. doi: 10.1139/m92-017.
The membrane fractions of the microaerobically grown type strains of Wolinella curva, Wolinella recta, Bacteroides ureolyticus, and Bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and CO-binding cytochrome c. Soluble cytochrome c and CO-binding cytochrome c were also present. Although B. gracilis is oxidase negative, it possessed cytochrome c. With H2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. With formate as the electron donor, the H+/O ratios of W. curva, W. recta, B. ureolyticus, and B. gracilis were 0.75, 1.66, 2.06, and 2.04, respectively. With H2 as the electron donor, the H+/O ratios of W. curva, B. ureolyticus, and B. gracilis were 1.25, 1.97, and 2.36, respectively. Proton translocation was inhibited by the protonophore carbonylcyanide m-chlorophenylhydrazone. The results confirm that the organisms are not anaerobes but are microaerophiles capable of respiring with oxygen.
弯曲沃林氏菌、直形沃林氏菌、解脲拟杆菌和纤细拟杆菌的微需氧生长型菌株的膜组分含有膜结合细胞色素b、细胞色素c和一氧化碳结合细胞色素c。也存在可溶性细胞色素c和一氧化碳结合细胞色素c。尽管纤细拟杆菌氧化酶阴性，但它含有细胞色素c。以氢气或甲酸盐作为电子供体时，向厌氧细胞中加入脉冲氧后会发生质子外流。以甲酸盐作为电子供体时，弯曲沃林氏菌、直形沃林氏菌、解脲拟杆菌和纤细拟杆菌的H⁺/O比率分别为0.75、1.66、2.06和2.04。以氢气作为电子供体时，弯曲沃林氏菌、解脲拟杆菌和纤细拟杆菌的H⁺/O比率分别为1.25、1.97和2.36。质子载体羰基氰化物间氯苯腙抑制质子转运。结果证实这些生物体不是厌氧菌，而是能够利用氧气进行呼吸的微需氧菌。
