Mohácsi A, Fülöp T, Kozlovszky B, Seres I, Leövey A
First Department of Internal Medicine, University Medical School of Debrecen, Hungary.
Clin Biochem. 1992 Aug;25(4):285-8. doi: 10.1016/0009-9120(92)80034-e.
It has been established that phagocytic cells are integral components of advanced arteriosclerotic plaques but their role in plaque formation remains unclear. Therefore, toxic agents, such as superoxide anion produced by polymorphonuclear leukocytes (PMNLs) were studied in a clinically defined group of arteriosclerotic patients suffering from obliterative arteriosclerosis of the lower legs. Owing to a close correlation between O2- generation and calcium, the intracellular free calcium concentrations of PMNLs were measured in a resting state and after stimulation with various agents, for example, opsonized zymosan (OZ), the chemotactic peptide f-met-leu-phe (FMLP), and the calcium ionophore A23187. Healthy aged-matched controls were employed. The patients were divided into two age groups: 30-59 years and 60-80 years. We found that in the younger group of arteriosclerotic patients, superoxide anion production and intracellular free calcium concentrations were increased even in the resting state, and only a slight increase was observed after stimulation compared with healthy controls. Granulocyte responses seemed to be similar, independent of the patient's age, to those found in healthy elderly subjects. Arteriosclerosis appears to be associated with an early aging process expressing marked alterations that are greater than those associated with normal aging.
业已确定，吞噬细胞是晚期动脉粥样硬化斑块的重要组成部分，但其在斑块形成中的作用仍不清楚。因此，我们在一组经临床确诊的患有下肢闭塞性动脉硬化的动脉粥样硬化患者中，研究了毒性因子，如多形核白细胞（PMNLs）产生的超氧阴离子。由于O2-生成与钙密切相关，我们测量了PMNLs在静息状态下以及在用各种试剂刺激后的细胞内游离钙浓度，例如调理酵母聚糖（OZ）、趋化肽f-甲硫-亮-苯丙氨酸（FMLP）和钙离子载体A23187。采用了年龄匹配的健康对照。患者被分为两个年龄组：30-59岁和60-80岁。我们发现，在较年轻的动脉粥样硬化患者组中，即使在静息状态下，超氧阴离子的产生和细胞内游离钙浓度也会增加，与健康对照相比，刺激后仅观察到轻微增加。粒细胞反应似乎与患者年龄无关，与健康老年受试者的反应相似。动脉粥样硬化似乎与早期衰老过程有关，表现出明显的改变，这些改变大于与正常衰老相关的改变。
