Lewis J W, Kliger D S
Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064.
J Bioenerg Biomembr. 1992 Apr;24(2):201-10. doi: 10.1007/BF00762678.
Much progress has been made in recent years toward understanding the interactions between various proteins responsible for visual transduction which are initiated by an activated state of visual pigments. However, the changes which take place in the visual pigments themselves to convert them to the activated state are more poorly understood. Many spectroscopic techniques have been applied to this problem in recent years and considerable progress has been made. A major goal of these efforts is to understand at which stages protein change occurs and to characterize its structural features. In the visual system evidence is accumulating, for example, that chromophore independent protein change begins immediately prior to lumirhodopsin formation. Considerable insight has been gained recently into the early intermediates of visual transduction and the stage is set to achieve similar understanding of the later intermediates leading to rhodopsin's activated state.
近年来，在理解负责视觉转导的各种蛋白质之间的相互作用方面取得了很大进展，这些相互作用是由视觉色素的激活状态引发的。然而，对于视觉色素自身为转变为激活状态而发生的变化，人们了解得更少。近年来，许多光谱技术已应用于这个问题，并取得了相当大的进展。这些研究工作的一个主要目标是了解蛋白质变化发生在哪些阶段，并描述其结构特征。例如，在视觉系统中，越来越多的证据表明，发色团独立的蛋白质变化在发光视紫红质形成之前就立即开始了。最近，人们对视觉转导的早期中间体有了相当深入的了解，并且已经为深入了解导致视紫红质激活状态的后期中间体奠定了基础。
