Krasnikova T L, Minchuna V P, Runikhin A Iu, Arabidze G G, Mazaev A V, Iudaev A D
Kardiologiia. 1992 Feb;32(2):36-40.
The effects of prostaglandin E2 (PGE2) infusions on lymphocyte beta 2-adrenoceptor-dependent adenylate cyclase (beta 2ARAC) system were studied in 26 patients with resistant hypertension (RH). The density of beta 2-adrenoceptors and their affinity for 1-isoproterenol were measured with 125ICYP and adenylate cyclase activity was determined by alpha-[32P]AMP generation in mononuclear lymphocytes of RH patients before and 1, 7, and 14 days after the last PGE2 infusion. Plasma epinephrine and norepinephrine concentrations were analyzed by using HPLC. The resistance to the four-component drug therapy was overcome with PGE2 infusions in patients, leading to a decrease in lymphocyte beta 2-adrenoceptor density and an increase in beta 2-receptor affinity for catecholamines and in adenylate cyclase activation by stimulating agents. PGE2 infusions unchanged the sensitivity to antihypertensive drugs in patients resulting in an increase in lymphocyte beta 2-adrenoceptor density, a decrease in beta 2-receptor affinity for catecholamines and in adenylate cyclase activation.
在26例顽固性高血压（RH）患者中研究了前列腺素E2（PGE2）输注对淋巴细胞β2 - 肾上腺素能受体依赖性腺苷酸环化酶（β2ARAC）系统的影响。在最后一次PGE2输注前以及输注后1天、7天和14天，用125I - CYP测定RH患者单核淋巴细胞中β2 - 肾上腺素能受体的密度及其对1 - 异丙肾上腺素的亲和力，通过α - [32P] AMP生成测定腺苷酸环化酶活性。采用高效液相色谱法分析血浆肾上腺素和去甲肾上腺素浓度。PGE2输注克服了患者对四联药物治疗的抵抗，导致淋巴细胞β2 - 肾上腺素能受体密度降低，β2 - 受体对儿茶酚胺的亲和力增加，以及刺激剂对腺苷酸环化酶的激活增加。PGE2输注未改变患者对抗高血压药物的敏感性，导致淋巴细胞β2 - 肾上腺素能受体密度增加，β2 - 受体对儿茶酚胺的亲和力降低，以及腺苷酸环化酶激活降低。
